Human Anatomy,

McKinley & O'Loughlin

First Edition

Chapter 21: Blood

Blood

Considered a connective tissue: contains

Cells a liquid ground substance (called plasma) dissolved protein fibers.

About four times more viscous (or thicker) than water. Temperature of blood is about 1C higher than measured body temperature.
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Blood

Whole blood can be separated:

Liquid component cellular components

machine called a centrifuge.

blood is withdrawn from a vein and collected in a centrifuge tube tube is placed into the centrifuge, which then spins it in a circular motion for several minutes rotational movement separates the blood into liquid and cellular components
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Components of Blood

Erythrocytes (or red blood cells)

form the lower layer of the centrifuged blood typically make up about 44% of a blood sample
makes up the middle layer thin, slightly gray-white layer composed of cells called leukocytes (or white blood cells) and cell fragments called platelets forms less than 1% of a blood sample straw-colored liquid that rises to the top generally makes up about 55% of blood
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Buffy coat

Plasma

Components of Blood

Erythrocytes and the components of the buffy coat are called the formed elements.

not cells, merely fragments broken off from a larger cell

Formed elements and the liquid plasma compose whole blood.

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Functions of Blood Transportation

Transports numerous elements and compounds throughout the body.

erythrocytes carry oxygen from the lungs to body cells and then transport carbon dioxide from the cells back to the lungs for expulsion from the body blood plasma

transports nutrients that have been absorbed from the GI tract hormones secreted by the endocrine organs to their target cells

plasma

carries waste products from the cells to organs such as the kidneys, where these waste products are removed

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Functions of Blood Regulation of Body Temp.

Regulates body temperature.

plasma absorbs and distributes heat throughout the body for cooling the blood vessels in the dermis dilate and dissipate the excess heat through the integument when the body needs to conserve heat, the dermal blood vessels constrict, and the warm blood is shunted to deeper blood vessels in the body
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Functions of Blood Regulation of pH Levels

pH is a measure of how alkaline or acidic a fluid is.

Neutral pH is measured at exactly 7. Acidic fluids (e.g., orange juice) are between 0 and 7. Alkaline fluids (e.g., milk) are between 7 and 14.

Blood plasma contains compounds and ions that may be distributed to the fluid among tissues (interstitial fluid) to help maintain normal tissue pH. Blood plasma pH is continuously regulated at a value of 7.4 the pH level required for normal cellular functioning.
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Functions of Blood Maintenance of Fluid Levels

Maintains normal fluid levels in the cardiovascular system. Prevents fluid loss. Constant exchange of fluid between the blood plasma and the interstitial fluid. If too much fluid is absorbed in the blood, high blood pressure results. If too much fluid escapes the bloodstream and enters the tissues, blood pressure drops to unhealthy low levels, and the tissues swell with excess fluid.
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Functions of Blood Maintenance of Fluid Levels

To maintain balance of fluid exchange between the blood and the interstitial fluid, blood contains compounds (such as salts and some proteins) to prevent excess fluid loss in the plasma.

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Functions of Blood Protection

Leukocytes (white blood cells) help guard against infection by mounting an immune response if a pathogen or an antigen is found. Plasma transports antibodies, which are molecules that can immobilize antigens until a leukocyte can completely kill or remove the antigen. Platelets and blood proteins protect the body against blood loss by forming blood clots on damaged vessels.

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Components of Plasma

Complex mixture of water, proteins, and other solutes. When the proteins are moved from plasma, the remaining fluid is termed serum. Water makes up about 92% of plasmas total volume.

water facilitates the transport of materials in the plasma

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Plasma Proteins

The next most abundant materials in plasma are the plasma proteins. Make up about 7% of the plasma.

6 and 8 grams of protein in a volume of 100 milliliters of blood (referred to as g/dl) albumins globulins fibrinogen regulatory proteins
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The plasma proteins include:

Plasma Proteins Albumins

Smallest and most abundant of the plasma proteins.

make up approximately 58% of total plasma proteins

Regulate water movement between the blood and interstitial fluid. Albumins act as transport proteins that carry ions, hormones, and some lipids in the blood.

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Plasma Proteins Globulins

Second largest group of plasma proteins, forming about 37% of all plasma proteins. Smaller alpha-globulins and the larger beta-globulins primarily bind, support, and protect certain waterinsoluble or hydrophobic molecules, hormones, and ions. Gamma-globulins: Also called immunoglobulins or antibodies. Produced by some of our defense cells to protect the body against pathogens that may cause disease.
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Plasma Proteins Fibrinogen

Makes up about 4% of all plasma proteins. Responsible for blood clot formation. Following trauma to the walls of blood vessels, fibrinogen is converted into long, insoluble strands of fibrin, which is the essence of a blood clot.

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Plasma Proteins Regulatory Proteins

Form a very minor class of plasma proteins.

<1% of total plasma proteins

Include enzymes to accelerate chemical reactions in the blood and hormones being transported throughout the body to target cells.

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Solutes

Plasma is an extracellular fluid (ECF). it includes all body fluids that are not found inside cells Plasma is somewhat like interstitial fluid, in that both have similar concentrations of nutrients, waste products, and electrolytes Concentration of dissolved oxygen is higher in plasma than in interstitial fluid, because the cells take up and use the oxygen from the interstitial fluid during energy production.

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Solutes

Difference in concentration ensures that oxygen will continue to diffuse from the blood into the tissues. Difference in concentration ensures that carbon dioxide will readily diffuse from the interstitial fluid into the blood, where it will be carried to the lungs and discharged from the body.
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Formed Elements in the Blood

Erythrocytes

make up more than 99% of formed elements primary function is to transport respiratory gases in the blood
make up less than .01% of formed elements contribute to defending the body against pathogens make up less than 1% of formed elements and help with blood clotting

Leukocytes

Platelets

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Hematocrit

Percentage of erythrocytes in the blood. Values vary slightly and are dependent on age and sex. Adult males range between 42% and 56% . Females range from 38% to 46%. Childrens hematocrit ranges also vary and differ from adult values. Altitude can affect the hematocrit. body compensates by making more erythrocytes more erythrocytes in the blood can carry more oxygen to the tissues

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Erythrocytes

Mature erythrocytes lack nuclei.

Transport oxygen and carbon dioxide to and from the tissues and the lungs. Lack of nuclei enables them to carry respiratory gases more efficiently.

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Hemoglobin in Erythrocytes

Every erythrocyte is filled with approximately 280 million molecules of a red-pigmented protein called hemoglobin. Transports oxygen and carbon dioxide, and is responsible for the characteristic bright red color of arterial blood. Hemoglobin that contains no oxygen has a deep red color that is perceived as blue because the blood within these veins is observed through the layers of the skin and the subcutaneous tissue.
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Hemoglobin in Erythrocytes

Each hemoglobin molecule consists of four protein building blocks, called globins.

Alpha (a) chains Beta (b) chains. ring shaped an iron (Fe) ion in its center.

All globin chains contain a nonprotein (or heme) group:

Oxygen binds to these iron ions for transport in the blood. Each hemoglobin molecule:

four iron ions is capable of binding four molecules of oxygen.

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Hemoglobin in Erythrocytes

Oxygen binding is fairly weak

ensures rapid attachment and detachment of oxygen with hemoglobin.

when the erythrocytes pass through the blood vessels of the lungs.

Oxygen binds to the hemoglobin

It leaves the hemoglobin

when the erythrocytes pass through the blood vessels of body tissues.

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Erythrocyte Life Cycle

No organelles, therefore can not sustain itself. Finite life span of about 120 days. Daily:

About 1% of oldest RBCs are removed By macrophages

Are phagocytized by liver and spleen.

Some components saved, some discarded

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Erythrocyte Life Cycle

Some components saved, some discarded

Heme group:

Converted to biliverdin (green pigment) Then converted to bilirubin

In bile, produced by liver Bile enters the digestive tract Helps emulsify fat Bilirubin modified and removed via urine and feces

Iron:

Transported by transferrin to liver

Transferred to ferritin for storage

Ferritin can be transported to red bone marrow

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Blood Types

Determined by membrane proteins in the RBC cell membrane. Called surface antigens (agglutinogens). Most common group: ABO blood group

Two antigens: A and B Type A: have the A surface antigen Type B: have the B surface antigen Type AB: have both the A and the B surface antigens Type O: have neither the A or the B surface antigen.
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ABO blood types:

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Blood Types

Antibodies (agglutinins) to the surface antigens are in the plasma ABO group has anti-A antibodies and anti-B antibodies

Type Type Type Type

A: have anti-B B: have anti-A AB: has neither anti-A or anti-B O: has both anti-A and anti- B
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Antibodies

An antibody interacts with a specific antigen. The ABO blood group has both anti-A and anti-B antibodies that react with the surface antigen A and the surface antigen B, respectively. The antibodies in your blood plasma do not recognize the surface antigens on your erythrocytes.

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Blood Types

Rh blood type

Based on another surface antigen Called either Rh or D

Rh positive (Rh+): has the antigen Rh negative (Rh-): does not have the antigen

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Blood Types

Rh blood type Antibody only present with exposure to Rh+ blood (the antigen) Only people with Rh- blood can have the antibody Erthryroblastosis fetalis: Rh+ antibodies in plasma of an Rh- mother can cross the placenta

RhoGAM: prevent antibody development

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Leukocytes

Leukocytes help initiate an immune response and defend the body against invading pathogens. Leukocytes are true cells in that they contain a nucleus and cellular organelles. Leukocytes also differ from erythrocytes in that they are about 1.5 to 3 times larger, and they do not contain hemoglobin.

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Leukocytes

The five types of leukocytes are divided into two distinguishable classesgranulocytes and agranulocytesbased upon the presence or absence of visible organelles termed specific granules.

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Leukocytes Granulocytes

Neutrophil

6070% of the total number of leukocytes

Eosinophils have reddish, or pink-orange granules in their cytoplasm.

constitute about 24% of the total number of leukocytes nucleus usually has two lobes, which are connected by a thin strand 1.5 times larger than erythrocytes least numerous of the granulocytes constitute about 0.51% of the total number of leukocytes always exhibit a bilobed nucleus and abundant blue-violet granules in the cytoplasm

Basophils are

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Leukocytes Agranulocytes

Agranulocytes are leukocytes that have such small granules in their cytoplasm that they are frequently overlooked upon casual observationhence the name agranulocyte. Agranulocytes include both lymphocytes and monocytes.

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Leukocytes Agranulocytes Lymphocytes

T-lymphocytes (T-cells)

manage and direct an immune response some directly attack foreign cells and virusinfected cells stimulated to become plasma cells and produce antibodies attack abnormal and infected tissue cells
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B-lymphocytes (B-cells)

Natural killer cells (NK cells)

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Leukocytes Agranulocytes Monocytes

Up to three times the diameter of an erythrocyte. Constitute about 38% of all leukocytes. Nucleus is kidney-shaped or U-shaped. Macrophages phagocytize bacteria, cell fragments, dead cells, and debris.

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Platelets

Irregular, membrane-enclosed cellular fragments

about 2 micrometers in diameter (less than onefourth the size of an erythrocyte).

In stained preparations, they exhibit a dark central region. Sometimes called thrombocytes.

Continually produced in the red bone marrow by cells called megakaryocytes.

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Platelets

Severe trauma to a blood vessel causes the blood to coagulate, or clot. Components in the plasma produce a web of fibrin that traps erythrocytes and platelets in the web to halt blood flow.

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Hemopoiesis

Also called hematopoiesis Production of the formed elements of blood Occurs in red bone marrow

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Hemopoiesis

Begins with hemopoietc stem cells Called Hemocytoblast

Pluripotent cells Produce two cell lines

Myloid line

Everything but lymphocytes lymphocytes

Lymphoid line

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Hemopoiesis

Influenced by a number of hormones and growth factors Colony-stimulating factors:

Multi-CSF: RBCs,granulocytes,monocytes, platelets

From myloid stem cell

From progenitor cell From myeloblast cells

GM-CSF: granulocytes, monocytes

G-CSF: granulocytes

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Hemopoiesis

Colony-stimulating factors: contined

M-CSF: monocytes

From monoblasts

Thrombopoietin: megakaryocytes and platelets Erythropoietin: hormone from the kidney, RBCs

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Hemopoiesis

Erythropoiesis: from progenitor cell

Reticulocyte: loses all organelles

Thrombopoiesis Leukopoiesis

Granulocyte maturation: from myeloblast Monocyte maturation: from monoblast Lymphocyte maturation: from lymphoid stem cell

B-lymphoblast T-lymphoblast
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